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Immunology and Infection
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一个流程粘附实验来研究白细胞招聘剪切应力的人力肝窦内皮细胞条件下
JoVE Journal
Immunology and Infection
This content is Free Access.
JoVE Journal
Immunology and Infection
A Flow Adhesion Assay to Study Leucocyte Recruitment to Human Hepatic Sinusoidal Endothelium Under Conditions of Shear Stress
Please note that all translations are automatically generated.
Click here for the English version.
一个流程粘附实验来研究白细胞招聘剪切应力的人力肝窦内皮细胞条件下
DOI:
10.3791/51330-v
•
11:38 min
•
March 21, 2014
•
Shishir Shetty
,
Christopher J. Weston
,
David H. Adams
,
Patricia F. Lalor
1
NIHR Biomedical Research Unit, Centre for Liver Research, School of Immunity and Infection
,
University of Birmingham
Chapters
00:05
Title
01:59
Cytokine Stimulation of Endothelial Cells
04:11
Isolation and Pretreatment of Peripheral Blood Lymphocytes
06:09
Setup of Flow Assay System
08:33
Flow AssayTechnique and Recording of Adhesion for Analysis
09:35
Results: Adhesion Cascade of Blood Lymphocytes over Hepatic Sinusoidal Endothelium
10:44
Conclusion
Summary
Automatic Translation
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Automatic Translation
白细胞募集到肝脏发生这是由独特的肝窦内皮细胞衬里肝窦的专业渠道内。整个生理切应力条件下的人力肝窦内皮细胞白细胞招聘相衬显微镜可以促进其背后这一过程的分子机制的阐明。
Tags
Flow Adhesion Assay
Leucocyte Recruitment
Human Hepatic Sinusoidal Endothelium
Shear Stress
Inflammatory Liver Diseases
Fibrosis
Cirrhosis
Molecular Mechanisms
Therapeutic Targets
Leucocyte Infiltration
Hepatic Sinusoids
Hepatic Sinusoidal Endothelial Cells (HSEC)
Adhesion Cascade
Tethering
Activation
Firm Adhesion
Crawling
Transmigration
Phase Contrast Microscopy
Inhibitors
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