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快速,特异微孔板检测为内和细胞外抗坏血酸在培养细胞的测定
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A Rapid and Specific Microplate Assay for the Determination of Intra- and Extracellular Ascorbate in Cultured Cells
Please note that all translations are automatically generated.
Click here for the English version.
快速,特异微孔板检测为内和细胞外抗坏血酸在培养细胞的测定
DOI:
10.3791/51322-v
•
11:56 min
•
April 11, 2014
•
Darius J. R. Lane
,
Alfons Lawen
1
Molecular Pharmacology and Pathology Program, Department of Pathology & Bosch Institute
,
University of Sydney
,
2
Department of Biochemistry and Molecular Biology, School of Biomedical Sciences
,
Monash University
Chapters
00:05
Title
02:01
Determine Intracellular Ascorbate in Cultured Cells
04:16
Determine the Amount of Ascorbate Released and Extracellular Ascorbate
06:25
Results: Determination of Intracellular and Extracellular Ascorbate in Cultured Cells
11:36
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
抗坏血酸发挥许多重要作用的细胞代谢,其中许多只明朗化的趋势。这里,我们描述一个介质通量,为在细胞培养物内和细胞外抗坏血酸的确定具体且廉价微孔板检测。
Tags
Ascorbate
Vitamin C
Microplate Assay
Intracellular
Extracellular
Cell Culture
Medium-throughput
High-sensitivity
Iron Metabolism
Neuroprotective
Neuromodulatory
Brain Homeostasis
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