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从海马CA1区片核富含分的分离，研究突触核信使蛋白质的活性依赖进口核
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Neuroscience
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JoVE Journal Neuroscience
Isolation of CA1 Nuclear Enriched Fractions from Hippocampal Slices to Study Activity-dependent Nuclear Import of Synapto-nuclear Messenger Proteins
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从海马CA1区片核富含分的分离，研究突触核信使蛋白质的活性依赖进口核

DOI:
10.3791/51310-v

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10:03 min

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August 10, 2014

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, , , ,
1RG Neuroplasticity,Leibniz Institute for Neurobiology, 2Department of Cell Biology,Utrecht University

Chapters

  • 00:05Title
  • 02:03Preparation of Hippocampal Slices
  • 03:13Baseline Recording and LTP Induction
  • 05:17Isolation of Nuclear Enriched Fractions from Hippocampal CA1
  • 07:29Semiquantitative Immunoblotting for Synapto-nuclear Proteins
  • 08:02Results: Induction of CA1 LTP Leads to the Accumulation of pJacS180 in the Nucleus
  • 08:53Conclusion

Summary

Automatic Translation

Automatic Translation

我们提供了用于诱导的长时程增强，在海马CA1区和核富集级分的从片的tetanized区域的随后分离的详细协议。这种方法可以被用来确定活性依赖的核蛋白质导入在学习和记忆的细胞模型。

Tags

Nuclear Enriched FractionHippocampal SlicesSynaptic PlasticityLong-term PotentiationLong-term DepressionProtein TranslocationImmunoblottingActivity-dependentSignaling CascadesCA1 NeuronsERK1/2Jacob

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