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设置了一个<em>体外</em大鼠血脑屏障>模型（BBB）：在BBB抗渗性和受体介导的运输为中心
JoVE Journal
Medicine
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JoVE Journal Medicine
Setting-up an In Vitro Model of Rat Blood-brain Barrier (BBB): A Focus on BBB Impermeability and Receptor-mediated Transport
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设置了一个<em>体外</em大鼠血脑屏障>模型（BBB）：在BBB抗渗性和受体介导的运输为中心

DOI:
10.3791/51278-v

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16:26 min

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June 28, 2014

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, , , ,
1VECT-HORUS SAS, 2Aix-Marseille Université,CNRS, NICN UMR 7259

Chapters

  • 00:05Title
  • 02:55Isolation of Rat Brain Microvessels
  • 09:27Purification and Proliferation of RBEC Primocultures
  • 10:32Differentiation: Setting up the In Vitro BBB Model
  • 14:50Results: Immunocytochemical Characterization of the Rat In Vitro BBB Model and the Paracellular Permeability of LY across the Endothelial Monolayer
  • 15:44Conclusion

Summary

Automatic Translation

Automatic Translation

本研究的目的是要验证的体外血脑屏障模型涉及的大鼠同源内皮细胞和星形胶质细胞的共培养物的再现性。内皮细胞单层呈现高跨膜电阻和低LY渗透性。具体TJ蛋白的表达，功能反应，炎症和运输者和受体的功能进行了评估。

Tags

In VitroRatBlood-brain BarrierPrimary Brain Endothelial CellsAstrocytesCo-cultureTight JunctionsTransendothelial Electrical ResistancePermeabilityP-glycoproteinTranscytosisReceptor-mediated Transport

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