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ATP合酶二聚体线粒体通过冷冻电子断层扫描可视化
JoVE Journal
Biology
This content is Free Access.
JoVE Journal
Biology
Visualization of ATP Synthase Dimers in Mitochondria by Electron Cryo-tomography
Please note that all translations are automatically generated.
Click here for the English version.
ATP合酶二聚体线粒体通过冷冻电子断层扫描可视化
DOI:
10.3791/51228-v
•
10:39 min
•
September 14, 2014
•
Karen M. Davies
,
Bertram Daum
,
Vicki A. M. Gold
,
Alexander W. Mühleip
,
Tobias Brandt
,
Thorsten B. Blum
,
Deryck J. Mills
,
Werner Kühlbrandt
1
Department of Structural Biology
,
Max Planck Institute of Biophysics
Chapters
00:05
Title
01:37
Preparation of Mitochondria for Electron Cryo-tomography
03:14
Recording Tomographic Tilt Series
05:20
Creation and Segmentation of Tomographic Volumes
06:25
Subtomogram Averaging of ATP Synthase Dimers and Fitting of X-Ray Structures
07:05
Results: Analysis of Mitochondrial Electron Cryo-tomograms
08:54
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
我们提出了如何收集和处理电子低温断层整个线粒体的协议。该技术提供了详细分析上市的结构,功能和组织的大的膜蛋白复合物中天然生物膜。
Tags
Electron Cryo-tomography
Structural Biology
3D Tomograms
Subtomogram Averaging
ATP Synthase
Mitochondria
Inner Membrane Cristae
Complex I
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