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Immunology and Infection
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评估小鼠浆细胞树突状细胞在淋巴集结的开发利用造血祖细胞的过继转移
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Immunology and Infection
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JoVE Journal
Immunology and Infection
Assessing the Development of Murine Plasmacytoid Dendritic Cells in Peyer’s Patches Using Adoptive Transfer of Hematopoietic Progenitors
Please note that all translations are automatically generated.
Click here for the English version.
评估小鼠浆细胞树突状细胞在淋巴集结的开发利用造血祖细胞的过继转移
DOI:
10.3791/51189-v
•
13:34 min
•
March 17, 2014
•
Haiyan S. Li
,
Stephanie S. Watowich
2
1
Department of Immunology
,
The University of Texas MD Anderson Cancer Center
,
2
The University of Texas Graduate School of Biomedical Sciences
Chapters
00:05
Title
01:56
Hydrodynamic Gene Transfer
02:54
Adoptive Transfer of Common Dendritic Progenitors (CDPs)
06:38
Isolation and Enumeration of Peyer’s Patch Plasmacytoid Dendritic Cells
09:36
Results: Analysis of PP pDC in
Ifnar
/-
Mice Upon Flt3L HGT and Adoptive Transfer of CPDs
13:12
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
这个协议描述的实验程序来评估浆细胞样树突细胞的Peyer氏从共同的树突状细胞前体细胞的补丁的分化,使用涉及FACS介导的细胞分离,水动力的基因转移,并在淋巴集结免疫子集流分析技术。
Tags
Murine Plasmacytoid Dendritic Cells
Peyer’s Patches
Adoptive Transfer
Hematopoietic Progenitors
Common Dendritic Cell Progenitors
Flt3 Ligand
Ifnar-/- Mice
Flow Cytometry
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