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翻译起始的分析在应力条件由多聚核糖体剖析
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JoVE Journal
Biology
Analysis of Translation Initiation During Stress Conditions by Polysome Profiling
Please note that all translations are automatically generated.
Click here for the English version.
翻译起始的分析在应力条件由多聚核糖体剖析
DOI:
10.3791/51164-v
•
10:59 min
•
May 19, 2014
•
Laëtitia Coudert*
1,2
,
Pauline Adjibade*
1,2
,
Rachid Mazroui
2
1
Department of Molecular Biology, Medical Biochemistry, and Pathology, Faculty of Medicine
,
Laval University
,
2
CHU de Quebec Research Center
Chapters
00:05
Title
01:45
Preparation of the Density Gradient Fraction System and Sucrose Gradients
03:04
Preparation of Cell Extracts
05:10
Loading Extracts onto Sucrose Gradients and Ultracentrifugation
06:03
Fractionation of Cytoplasmic Extracts for Polysome Profiling
06:46
Extraction of Protein and RNA for Analysis
08:45
Results: Polysome Profiling Detects Changes in Translation Initiation in Response to Stress Conditions
10:01
Conclusion
Summary
Automatic Translation
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Automatic Translation
在这里,我们描述一个变化来分析真核细胞中的mRNA的翻译的响应于胁迫条件的启动方法。此方法是基于换算从非翻译的核糖体的核糖体的蔗糖梯度的速度分离。
Tags
Polysome Profiling
Translation Initiation
Stress Conditions
Eukaryotic Cells
MRNA Translation
Density Gradient Fractionation
Ribosome Populations
Translational Control
Neurodegenerative Diseases
Cancer Development
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