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Immunology and Infection
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定量检测抗体诱导补体激活的方法对红血细胞
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Immunology and Infection
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JoVE Journal
Immunology and Infection
Methods for Quantitative Detection of Antibody-induced Complement Activation on Red Blood Cells
Please note that all translations are automatically generated.
Click here for the English version.
定量检测抗体诱导补体激活的方法对红血细胞
DOI:
10.3791/51161-v
•
06:29 min
•
January 29, 2014
•
Elisabeth M. Meulenbroek
,
Diana Wouters
,
Sacha Zeerleder
2
1
Department of Immunopathology, Sanquin Research and Landsteiner Laboratory Academic Medical Center
,
University of Amsterdam
,
2
Department of Hematology, Academic Medical Center
,
University of Amsterdam
Chapters
00:05
Title
01:21
Complement Deposition Analysis and Quantification of Hemolysis
04:34
Results: Quantitative Detection of Antibody-induced Complement Activation on RBCs
06:02
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
在这里,我们描述了两个测定法用于测定补体活化诱导的抗红细胞。过电流测定法的主要优点是它们的数量和易于理解的性质。
Tags
Antibody
Complement Activation
Red Blood Cells
RBC
Alloantibodies
Autoantibodies
Autoimmune Hemolytic Anemia
AIHA
Coombs Test
Hemolytic Assay
C3 Deposition
C4 Deposition
FACS Analysis
Quantitative Hemolytic Assay
Spectrophotometry
Hemoglobin
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