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快速合成及其与绿色荧光蛋白为基础的记者化学激活的转录因子筛选
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Rapid Synthesis and Screening of Chemically Activated Transcription Factors with GFP-based Reporters
Please note that all translations are automatically generated.
Click here for the English version.
快速合成及其与绿色荧光蛋白为基础的记者化学激活的转录因子筛选
DOI:
10.3791/51153-v
•
09:22 min
•
November 26, 2013
•
R. Scott McIsaac*
1,3
,
Benjamin L. Oakes*
1
,
David Botstein
2
,
Marcus B. Noyes
1
The Lewis-Sigler Institute for Integrative Genomics
,
Princeton University
,
2
Department of Molecular Biology
,
Princeton University
,
3
Division of Chemistry and Chemical Engineering
,
California Institute of Technology
Chapters
00:05
Title
01:33
Creation of Artificial Transcription Factors (ATFs)
02:28
Creation of ATF Plasmid Reporter
03:57
Quantify the Effect of ATF Induction on Gene Expression Using GFP Reporter
05:33
Quantify the Effect of the ATF Induction on Cell Growth
06:42
Create Inducible Genomic Alleles
07:20
Results: Analysis of GFP Induction by ATF-promoter Pairs
08:39
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
本协议描述了实验过程的人工转录因子(申请进度查询设施)与绿色荧光蛋白同源记者和通过流式细胞仪,刺激GFP表达的申请进度查询设施能力量化的快速建设。
Tags
Synthetic Biology
Transcription Factors
Gene Expression
Reporter Systems
GFP
Homologous Recombination
Artificial Transcription Factors
Conditional Gene Expression
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