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Immunology and Infection
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有效生成荧光标记疫苗病毒蛋白的方法
JoVE Journal
Immunology and Infection
This content is Free Access.
JoVE Journal
Immunology and Infection
Methodology for the Efficient Generation of Fluorescently Tagged Vaccinia Virus Proteins
Please note that all translations are automatically generated.
Click here for the English version.
有效生成荧光标记疫苗病毒蛋白的方法
DOI:
10.3791/51151-v
•
09:27 min
•
January 17, 2014
•
N. Bishara Marzook*
1
,
Dean J. Procter*
1
,
Helena Lynn
,
Yui Yamamoto
,
Jacquelyn Horsington
,
Timothy P. Newsome
1
School of Molecular Bioscience
,
University of Sydney
,
2
Department of Medicine
,
Center for Vascular Research
,
3
Asia Pacific Centre for Animal Health, Faculty of Veterinary Science
,
University of Melbourne
Chapters
00:05
Title
01:34
Creating the Recombinant Vector
02:48
Recombinant Virus Generation
05:26
Plaque Assay: NO Selection
05:59
Purity of Viral Stocks: PCR Screening of Genomic DNA
07:23
Results: Recombinant Vectors and the VACV Genome
08:43
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
此处描述了一种快速和模块化的协议,用于生成重组疫苗病毒,同时使用瞬时主导选择的方法表达荧光标记的蛋白质。
Tags
Fluorescent Protein Tagging
Vaccinia Virus
Recombinant Virus Generation
Homologous Recombination
Transient Dominant Selection
Metabolic Selection
Live-cell Microscopy
Virus-host Interactions
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