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新的细胞内病原体由Amoebal共培养和Amoebal富集途径探索
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Immunology and Infection
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JoVE Journal Immunology and Infection
Discovery of New Intracellular Pathogens by Amoebal Coculture and Amoebal Enrichment Approaches
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新的细胞内病原体由Amoebal共培养和Amoebal富集途径探索

DOI:
10.3791/51055-v

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09:04 min

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October 27, 2013

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, , ,
1Institute of Microbiology,University Hospital Center and University of Lausanne

Chapters

  • 00:05Title
  • 01:32Amoebal Coculture
  • 04:14Bacterial Staining and Immunofluorescence
  • 06:03Amoebal Enrichment
  • 07:10Representative Amoebal Coculture and Enrichment Results
  • 08:13Conclusion

Summary

Automatic Translation

Automatic Translation

Amoebal共存是使用贴壁变形虫以选择性地生长细胞内的病原体能够抵抗吞噬细胞如变形虫和巨噬细胞的细胞培养系统。因此，它代表发现新的传染性病原体的关键工具。 Amoebal富集允许发现其特定的细胞内细菌的新amoebal品种和。

Tags

Intracellular PathogensLegionellaMycobacteriaChlamydia-like OrganismsAmoebaeAmoebal CocultureAmoebal EnrichmentIsolationCharacterizationClinical SamplesEnvironmental SamplesBacterial GrowthPhagocytosisMacrophagesPathogenic

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