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内质网子域的培养细胞中的可视化

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Biology

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JoVE Journal Biology

Visualization of Endoplasmic Reticulum Subdomains in Cultured Cells

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内质网子域的培养细胞中的可视化

DOI:

10.3791/50985-v

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16:43 min

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February 18, 2014

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Matteo Fossati^2,3, Nica Borgese^3,4, Sara Francesca Colombo³, Maura Francolini²

¹Fondazione Filarete, ²Department of Biotechnology and Translational Medicine,University of Milan, ³Neuroscience Institute,National Research Council (CNR), ⁴Department of Health Science,“Magna Graecia” University of Catanzaro

Chapters

00:05Title
01:37Plasmid, Cell Culture, and Transfection with ER Fluorescent Proteins
03:21Live Fluorescence Scanning Confocal Microscopy
08:35Ultrastructural Analysis by Means of Transmission Electron Microscopy
12:38Representative Imaging Results
16:04Conclusion

Summary

Automatic Translation

我们描述了使用调查转染荧光蛋白通过活细胞的共聚焦成像装置驻留在内质网（ER）的分布和流动的成像方法。我们还超微结构分析其表达对这个亚细胞区室的结构的效果。

Tags

Endoplasmic Reticulum Cell Compartments Protein Trafficking Lipid Mobility FRAP FLIP Live-cell Imaging Fluorescence Microscopy Electron Microscopy Organelle Subcompartments Physiological Conditions Pathological Conditions

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