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潜在土壤胞外酶活性的高通量荧光测量
JoVE Journal
Environment
This content is Free Access.
JoVE Journal
Environment
High-throughput Fluorometric Measurement of Potential Soil Extracellular Enzyme Activities
Please note that all translations are automatically generated.
Click here for the English version.
潜在土壤胞外酶活性的高通量荧光测量
DOI:
10.3791/50961-v
•
12:33 min
•
November 15, 2013
•
Colin W. Bell
,
Barbara E. Fricks
,
Jennifer D. Rocca
,
Jessica M. Steinweg
,
Shawna K. McMahon
,
Matthew D. Wallenstein
1
Natural Resource Ecology Laboratory
,
Colorado State University
,
2
Biosciences Division
,
Oak Ridge National Laboratory
,
3
Department of Bioengineering
,
University of Colorado
Chapters
00:05
Title
02:18
Assay Set-up
04:00
Incubation of Assay Plates
04:56
Fluorescent Measurements on a Microplate Fluorometer
05:43
Data Analysis
07:03
Results: Analysis of Extracellular Enzyme Activity (EEA)
11:05
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
测量土壤胞外酶活性的潜在利率,绑定到一个荧光染料的合成底物加入到土壤样品。酶的活性被测量为荧光染料从基板释放的酶催化反应,其中更高的荧光指示多底物降解。
Tags
Soil Extracellular Enzyme Activities
Fluorometric Measurement
Soil Microbial Enzyme Activity
Fluorescence Enzyme Assay
Soil Slurries
Fluorescently Labeled Substrates
Potential Enzyme Activity
Soil Ecosystem Functional Dynamics
Enzyme Kinetics
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