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Aislamiento de células dendríticas mieloides y células epiteliales de timo humano
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1Department of General Neurology,Hertie Institute for Clinical Brain Research, 2Institute of Pharmacology,University of Bern, 3Department of Immunology,University Medical Center Hamburg-Eppendorf, 4Department of Thoracic and Cardiovascular Surgery,University Clinic Tuebingen, 5Department of Neurology,University Hospital Erlangen
Chapters
- 00:05Title
- 01:11Preparation of the Tissue
- 02:45Typical Enzymatic Tissue Digestion to Obtain a Single Cell Suspension
- 04:35Enrichment of Low Density Fraction (LDF) of Cells Using Percoll Density Separation
- 06:00Results: Representative Myeloid DC and TEC Populations Isolated from the Human Thymus
- 08:06Conclusion
Este protocolo detalla un método para aislar células presentadoras de antígeno de timo humano a través de diferentes etapas de la digestión enzimática del tejido seguido por centrifugación de densidad de la suspensión de células individuales y la clasificación finalmente magnético y / o FACS de las poblaciones de células de interés.
