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人中性粒细胞迁移的跨越培养膀胱上皮定量评价

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Immunology and Infection

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JoVE Journal Immunology and Infection

Quantitative Assessment of Human Neutrophil Migration Across a Cultured Bladder Epithelium

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人中性粒细胞迁移的跨越培养膀胱上皮定量评价

DOI:

10.3791/50919-v

•

11:20 min

•

November 07, 2013

•

Megan E. Lau, David A. Hunstad²

¹Department of Pediatrics,Washington University School of Medicine, ²Department of Molecular Microbiology,Washington University School of Medicine

Chapters

00:05Title
01:27Seeding and Growing 5637 Cells on Permeable Supports |
03:46Isolation of Human Neutrophils from Peripheral Blood
06:22Transuroepithelial Neutrophil Migration Assay
09:56Results: PMN Migration Across Bladder Epithelia in Response to Various Stimuli
10:59Conclusion

Summary

Automatic Translation

我们开发了一个体外模型，模仿与肾盂肾炎大肠杆菌膀胱感染，在急性炎症反应的重要组成部分。该transuroepithelial中性粒细胞迁移测定法使人类中性粒细胞迁移的定量评估跨越膀胱上皮，在可渗透载体培养中，响应于细菌感染或细胞趋化物质。

Tags

Neutrophil Migration Bladder Epithelium Urinary Tract Infection Uropathogenic Escherichia Coli Innate Immune Response Boyden Chamber Transuroepithelial Migration Assay Chemoattractant Eukaryotic Cells PMN Traversal

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