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提取与分析皮质醇从人与猴的毛
JoVE Journal
Biology
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JoVE Journal
Biology
Extraction and Analysis of Cortisol from Human and Monkey Hair
Please note that all translations are automatically generated.
Click here for the English version.
提取与分析皮质醇从人与猴的毛
DOI:
10.3791/50882-v
•
08:38 min
•
January 24, 2014
•
Jerrold Meyer
2
,
Melinda Novak
2
,
Amanda Hamel
,
Kendra Rosenberg
1
Department of Psychology
,
University of Massachusetts, Amherst
,
2
Neuroscience and Behavior Program
,
University of Massachusetts, Amherst
Chapters
00:05
Title
01:38
Sample Washing
02:15
Sample Grinding: Method 1 (Large Samples)
02:55
Cortisol (CORT) Extraction: Method 1 (Large Samples)
03:41
Sample Grinding: Method 2 (Small Samples)
04:24
CORT Extraction: Method 2 (Small Samples)
05:14
Solvent Evaporation, Sample Reconstitution, and CORT Assay
05:51
Results: Representative CORT Extraction Analysis for Human Hair Samples
08:01
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
皮质醇(CORT)积聚在人类和非人类灵长类动物的生长的头发轴。我们描述了用于提取和分析头发CORT以高精度和灵敏度的方法。是特别适合用于评估慢性压力过周时期到几个月头发CORT的测量。
Tags
Keyword Extraction:Cortisol
Hair
Extraction
Analysis
Rhesus Monkey
Human Scalp
HPA System
Biomarker
Stress
Enzyme Immunoassay
Methanol
Protein Matrix
Macaque Monkeys
Marmosets
Dogs
Polar Bears
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