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分离,培养和移植肌卫星细胞
JoVE Journal
Biology
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JoVE Journal
Biology
Isolation, Culture, and Transplantation of Muscle Satellite Cells
Please note that all translations are automatically generated.
Click here for the English version.
分离,培养和移植肌卫星细胞
DOI:
10.3791/50846-v
•
10:25 min
•
April 08, 2014
•
Norio Motohashi
,
Yoko Asakura
,
Atsushi Asakura
1
Stem Cell Institute, Paul and Sheila Wellstone Muscular Dystrophy Center, Department of Neurology
,
University of Minnesota Medical School
Chapters
00:05
Title
02:07
Isolation of Mononuclear Cells from Mouse Skeletal Muscle
04:19
Antibody Staining and Separation with MACS
06:39
Myoblast Differentiation and Transplantation for Skeletal Muscle Regeneration
08:50
Results: Representative Muscle Satellite Cell Transplantation
09:52
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
静止卫星细胞的纯群体的分离和培养,肌肉干细胞群,是必不可少的肌肉干细胞生物学和再生,以及干细胞移植在肌营养不良症和其他退行性疾病的治疗方法的理解。
Tags
Muscle Satellite Cells
Skeletal Muscle
Regeneration
Myoblast Transplantation
Muscle Stem Cells
Cell Isolation
MACS
FACS
Muscular Dystrophy
Cell Therapy
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