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体外 共生分析评估病原体诱导中性粒细胞跨上皮迁移
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Immunology and Infection
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JoVE Journal Immunology and Infection
In vitro Coculture Assay to Assess Pathogen Induced Neutrophil Trans-epithelial Migration
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体外 共生分析评估病原体诱导中性粒细胞跨上皮迁移

DOI:
10.3791/50823-v

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14:14 min

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January 06, 2014

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, , ,
1Department of Pediatrics,Harvard Medical School, 2Department of Pediatric Gastroenterology,MGH for Children, 3Mucosal Immunology and Biology Research Center,Massachusetts General Hospital

Chapters

  • 00:05Title
  • 02:01Collagen Coating Transwells
  • 03:25Seeding Collagen Coated Transwells with Epithelial Cells
  • 04:30Preparation of Epithelial Cell Layers for Migration Assay
  • 06:01Neutrophil Trans-epithelial Migration Assay
  • 11:27Results: Assessing Neutrophil Trans-epithelial Migration
  • 13:23Conclusion

Summary

Automatic Translation

Automatic Translation

中性粒细胞跨上皮迁移，以应对粘膜细菌感染，有助于上皮损伤和临床疾病。已经开发出一种 体外 模型，结合了病原体、人类嗜中性粒细胞和在转井过滤器上生长的极化人类上皮细胞层，以促进研究，以解开协调这一现象的分子机制。

Tags

In Vitro CocultureNeutrophil Trans-epithelial MigrationMucosal ImmunityPathogenic InfectionPolarized Epithelial MonolayerNeutrophil InfiltrationInflammatory ResponseTherapeutic Targets

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