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Immunology and Infection
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使用点击化学测量的影响,病毒感染宿主细胞RNA合成
JoVE Journal
Immunology and Infection
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JoVE Journal
Immunology and Infection
Using Click Chemistry to Measure the Effect of Viral Infection on Host-Cell RNA Synthesis
Please note that all translations are automatically generated.
Click here for the English version.
使用点击化学测量的影响,病毒感染宿主细胞RNA合成
DOI:
10.3791/50809-v
•
09:35 min
•
August 09, 2013
•
Birte Kalveram
,
Olga Lihoradova
,
Sabarish V. Indran
,
Jennifer A. Head
,
Tetsuro Ikegami
1
Department of Pathology, Sealy Center for Vaccine Development, Center for Biodefense and Emerging Infectious Diseases
,
University of Texas Medical Branch
Chapters
00:05
Title
01:16
Analysis of Transcription by Fluorescence Microscopy
04:16
Analysis of Transcription by Flow Cytometry
06:08
Infecting 293 Cells with MP-12
08:52
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
这个方法描述了使用的点击化学感染裂谷热病毒(RVFV)MP-12菌株后,在宿主细胞的转录变化来衡量。结果可以可视化,通过荧光显微镜定性或定量地获得通过流式细胞仪。此方法是适应性强,用于与其他病毒。
Tags
Click Chemistry
Viral Infection
Host-cell RNA Synthesis
Transcriptional Suppression
Rift Valley Fever Virus
5-ethynyluridine
Fluorescent Azide
Flow Cytometry
Fluorescence Microscopy
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