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协议用于实现<em>大肠杆菌</em>基于TX-TL无细胞表达系统合成生物学
JoVE Journal
Biology
This content is Free Access.
JoVE Journal
Biology
Protocols for Implementing an
Escherichia coli
Based TX-TL Cell-Free Expression System for Synthetic Biology
Please note that all translations are automatically generated.
Click here for the English version.
协议用于实现<em>大肠杆菌</em>基于TX-TL无细胞表达系统合成生物学
DOI:
10.3791/50762-v
•
16:11 min
•
September 16, 2013
•
Zachary Z. Sun*
1
,
Clarmyra A. Hayes*
2
,
Jonghyeon Shin
,
Filippo Caschera
,
Richard M. Murray
,
Vincent Noireaux
1
Department of Biology
,
California Institute of Technology
,
2
Department of Bioengineering
,
California Institute of Technology
,
3
Synthetic Biology Center, Department of Bioengineering
,
Massachusetts Institute of Technology
,
4
School of Physics and Astronomy
,
University of Minnesota
Chapters
00:05
Title
01:41
Cell Lysis
06:29
Extract Clarification
08:32
Experimental Execution of a TX-TL Reaction
10:40
Results: Assessment of TX-TL Cell-free Expression System
15:56
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
这次为期五天的协议列出了所有步骤,设备,以及用于创建和运行基于一个有效的内源性
大肠杆菌
从头TX-TL无细胞表达系统必要的补充软件。用试剂,该协议需要8个小时或更短设置一个反应,收集和处理数据。
Tags
Cell-free Expression System
Escherichia Coli
TX-TL
Transcription-translation
Synthetic Biology
Protein Expression
In Vitro
Endogenous
T7 RNA Polymerase
Lac/tet Repressors
MRNA Degradation
DNA Degradation
Biomolecular Breadboard
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