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Journal
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Immunology and Infection
/
在纯化造血干/祖细胞识别DNA突变
JoVE Journal
Immunology and Infection
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JoVE Journal
Immunology and Infection
Identifying DNA Mutations in Purified Hematopoietic Stem/Progenitor Cells
Please note that all translations are automatically generated.
Click here for the English version.
在纯化造血干/祖细胞识别DNA突变
DOI:
10.3791/50752-v
•
11:06 min
•
February 24, 2014
•
Ziming Cheng
,
Ting Zhou
2
,
Azhar Merchant
,
Thomas J. Prihoda
,
Brian L. Wickes
5
,
Guogang Xu
,
Christi A. Walter
5
,
Vivienne I. Rebel
2,5
1
Greehey Children’s Cancer Research Institute
,
UT Health Science Center at San Antonio
,
2
Department of Cellular and Structural Biology
,
UT Health Science Center at San Antonio
,
3
Department of Pathology
,
UT Health Science Center at San Antonio
,
4
Department of Microbiology
,
UT Health Science Center at San Antonio
,
5
Cancer Therapy and Research Center
,
UT Health Science Center at San Antonio
Chapters
00:05
Title
01:45
Isolation of Genomic DNA
04:10
Packaging of Genomic DNA
05:21
Plating Packaged Genomic DNA
07:07
Determination of a Putative Mutant Frequency
08:37
Results: The
in vivo
Mutagenesis Assay
10:35
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
这里我们描述了对小数目使用
了LacI
转基因小鼠模型纯化造血干细胞的
体内
诱变法。该
LacI的
基因可以分离来确定频率,位置和类型的DNA突变的自发产生或暴露于基因毒素后。
Tags
DNA Mutations
Hematopoietic Stem Cells
Hematopoietic Progenitor Cells
Genomic Instability
LacI Transgenic Mice
In Vivo Mutagenesis Assay
Lin-IL7R-Sca-1+cKit++ (LSK) Cells
DNA Sequencing
Mutation Frequency
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