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的代谢活性细菌在通才的肠道鉴定<em>海灰翅夜蛾</em>通过DNA的稳定同位素探测使用<sup> 13</sup> C-葡萄糖
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JoVE Journal
Biology
Identification of Metabolically Active Bacteria in the Gut of the Generalist
Spodoptera littoralis
via DNA Stable Isotope Probing Using
13
C-Glucose
Please note that all translations are automatically generated.
Click here for the English version.
的代谢活性细菌在通才的肠道鉴定<em>海灰翅夜蛾</em>通过DNA的稳定同位素探测使用<sup> 13</sup> C-葡萄糖
DOI:
10.3791/50734-v
•
12:11 min
•
November 13, 2013
•
Yongqi Shao*
1
,
Erika M Arias-Cordero*
1
,
Wilhelm Boland
1
Department of Bioorganic Chemistry
,
Max Planck Institute for Chemical Ecology
Chapters
00:05
Title
02:35
13
C-Labeling of DNA in Metabolically Active Gut Bacteria
03:42
Insect Dissection
05:06
DNA Extraction and Amplification
06:13
CsCl Gradient/DNA Ultracentrifugation
08:27
Processing the CsCl Gradients
09:45
Identifying Metabolically Active Gut Bacteria
11:13
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
与
海灰翅夜蛾
的肠道相关活动的细菌群落,由稳定同位素探测(SIP)耦合到焦磷酸测序确定。使用这种方法,识别社区内的代谢活性细菌种类与高的分辨率和精度完成。
Tags
Stable Isotope Probing
13C-glucose
Metabolically Active Bacteria
Spodoptera Littoralis
Insect Gut
Microbial Community
Pyrosequencing
Culture-independent Techniques
DNA Density Gradient Centrifugation
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