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打印温敏反向模具图案创作的双组分水凝胶的三维细胞培养
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Immunology and Infection
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JoVE Journal Immunology and Infection
Printing Thermoresponsive Reverse Molds for the Creation of Patterned Two-component Hydrogels for 3D Cell Culture
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打印温敏反向模具图案创作的双组分水凝胶的三维细胞培养

DOI:
10.3791/50632-v

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10:49 min

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July 10, 2013

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, , ,
1Department of Health Science & Technology,Cartilage Engineering & Regeneration, 2Biomaterials Department,Innovent e.V.

Chapters

  • 00:05Title
  • 01:36Preparation of the Poloxamer 407 Solution
  • 02:58Preparation of the 3D Printer
  • 04:24Optimization of the Printing Parameters
  • 06:42Printing and Elution of the Reverse Mold and Filling of the Voids
  • 09:38Results: Patterned Two-component Hydrogel Printing
  • 10:21Conclusion

Summary

Automatic Translation

Automatic Translation

àbioprinter被用于创建图案的水凝胶的基础上牺牲的模具。泊洛沙姆模具与第二水凝胶回填，然后洗脱，留下空隙充满了三分之一的水凝胶。此方法使用快速的洗脱和良好的印刷泊洛沙姆产生复杂的生物大分子结构。

Tags

BioprintingThermoresponsiveReverse MoldTwo-component Hydrogel3D Cell CulturePoloxamerAgaroseAlginate MethacrylateFITC-fibrinogenExtrusion BioprintingSacrificial MoldVascular NetworkNerve Guide Conduit

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