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在斑马鱼幼虫的高通量自动化的行为分析
JoVE Journal
Neuroscience
This content is Free Access.
JoVE Journal
Neuroscience
Automated High-throughput Behavioral Analyses in Zebrafish Larvae
Please note that all translations are automatically generated.
Click here for the English version.
在斑马鱼幼虫的高通量自动化的行为分析
DOI:
10.3791/50622-v
•
09:28 min
•
July 04, 2013
•
Holly Richendrfer
,
Robbert Créton
1
Department of Molecular Biology, Cell Biology and Biochemistry
,
Brown University
Chapters
00:05
Title
01:48
Collection of Zebrafish Embryos and Raising Larvae
03:08
Preparing Molds for Behavioral Analyses
04:02
Preparation of Imaging Cabinet
04:39
Image Capture
06:08
Image Analysis
07:31
Results: Example of Behaviors that Can Be Quantified in the High-throughput Assay
08:57
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
本实验室开发了一种新的高通量自动化的成像系统,是有用的,用于检测的几个不同的行为,在7天龄的斑马鱼幼虫。该系统可用于检测幼虫后已暴露于环境的有毒物质或药品的行为的微妙的变化,。
Tags
High-throughput
Behavioral Analysis
Zebrafish Larvae
Spontaneous Behavior
Aversive Stimulus
ImageJ
Swim Speed
Thigmotaxis
Avoidance Behavior
Environmental Toxicants
Pharmaceuticals
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