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Immunology and Infection
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四环素调节细胞株产生高滴度的慢病毒载体,专门针对树突状细胞
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Immunology and Infection
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JoVE Journal
Immunology and Infection
A Tetracycline-regulated Cell Line Produces High-titer Lentiviral Vectors that Specifically Target Dendritic Cells
Please note that all translations are automatically generated.
Click here for the English version.
四环素调节细胞株产生高滴度的慢病毒载体,专门针对树突状细胞
DOI:
10.3791/50606-v
•
16:04 min
•
June 19, 2013
•
Paul D. Bryson
,
Chupei Zhang
,
Chi-Lin Lee
,
Pin Wang
1
Mork Family Department of Chemical Engineering and Materials Science, Viterbi School of Engineering
,
University of Southern California, Los Angeles
Chapters
00:05
Title
01:59
Generating the GPRS Packaging Cell Line: A Tet-dependent SVGmu Cell Line
05:33
Constructing DC-LV Producer Cells by Concatemer Array Transfection
09:14
Evaluating Viral Production of Each Cell Clone
10:54
Producing and Concentrating Lentiviral Vectors
12:16
Results: Isolation of High-titer Lentiviral Vectors that Specifically Target Dendritic Cells
15:15
Conclusion
Summary
Automatic Translation
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Automatic Translation
这里,我们用逆转录病毒转导和concatemeric的转染建立的细胞系中能表达的慢病毒载体(LV)的组件在没有四环素。此LV编码绿色荧光蛋白是一种糖蛋白,SVGmu,这是具体的树突状细胞上的受体与假。
Tags
Lentiviral Vectors
High-titer
Dendritic Cells
Tet-off
Producer Cell Line
Concatemeric DNA Transfection
Reporter Gene
Transduction
T Cell Immune Response
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