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Immunology and Infection
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使用的细胞图像分析仪量化致病真菌与宿主细胞协会
JoVE Journal
Immunology and Infection
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JoVE Journal
Immunology and Infection
Use of Image Cytometry for Quantification of Pathogenic Fungi in Association with Host Cells
Please note that all translations are automatically generated.
Click here for the English version.
使用的细胞图像分析仪量化致病真菌与宿主细胞协会
DOI:
10.3791/50599-v
•
07:58 min
•
June 19, 2013
•
Charlotte Berkes
2
,
Leo Li-Ying Chan
3
,
Alisha Wilkinson
2,3
,
Benjamin Paradis
3
1
Department of Biology
,
Merrimack College
,
2
Center for Biotechnology and Biomedical Sciences
,
Merrimack College
,
3
Department of Technology R&D
,
Nexcelom Bioscience LLC
Chapters
00:05
Title
01:39
Infection of Macrophages with H. capsulatum, C. albicans
02:21
Macrophage Lysis
02:56
CFU Plating
03:28
Visualization of Fungi within Live Macrophages
03:54
Image Cytometric Analysis
06:04
Results: H. capsulatum in Macrophage Cells
07:12
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
在这里,我们将演示如何图像流式细胞仪可用于量化致病真菌与宿主细胞培养。 CFU枚举作为一种替代,可以使用这种技术。
Tags
Image Cytometry
Pathogenic Fungi
Candida Albicans
Histoplasma Capsulatum
Cryptococcus Neoformans
Macrophages
Host Cells
Colony Forming Unit
Flow Cytometry
Acridine Orange
Propidium Iodide
GFP-expressing Candida Albicans
Quantification
Cellular Pathogenesis
Infection
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