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使用的细胞图像分析仪量化致病真菌与宿主细胞协会
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Use of Image Cytometry for Quantification of Pathogenic Fungi in Association with Host Cells
DOI:

07:58 min

June 19, 2013

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Chapters

  • 00:05Title
  • 01:39Infection of Macrophages with H. capsulatum, C. albicans
  • 02:21Macrophage Lysis
  • 02:56CFU Plating
  • 03:28Visualization of Fungi within Live Macrophages
  • 03:54Image Cytometric Analysis
  • 06:04Results: H. capsulatum in Macrophage Cells
  • 07:12Conclusion

Summary

Automatic Translation

在这里,我们将演示如何图像流式细胞仪可用于量化致病真菌与宿主细胞培养。 CFU枚举作为一种替代,可以使用这种技术。

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