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Neuroscience
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胰蛋白酶精华协议分析视网膜血管小鼠模型
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Neuroscience
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JoVE Journal
Neuroscience
Trypsin Digest Protocol to Analyze the Retinal Vasculature of a Mouse Model
Please note that all translations are automatically generated.
Click here for the English version.
胰蛋白酶精华协议分析视网膜血管小鼠模型
DOI:
10.3791/50489-v
•
09:03 min
•
June 13, 2013
•
Jonathan C. Chou
,
Stuart D. Rollins
,
Amani A. Fawzi
1
Department of Ophthalmology
,
Northwestern University Feinberg School of Medicine
Chapters
00:05
Title
01:34
Retinal Preparation
02:22
Water Washes and Trypsin Digest
03:33
Separating the Vasculature
05:32
Flat-mounting the Vasculature
06:13
Results: Representative Images of Trypsin Digest
08:07
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
胰蛋白酶的消化是一个最常用的方法来分析视网膜血管。这份手稿中详细介绍的方法,包括关键的改动,优化技术,并删除无血管组织,同时保持整体架构的船只。
Tags
Retinal Vasculature
Trypsin Digest
Mouse Model
Flat-mount Retinal Blood Vessels
Retinal Vascular Changes
Microaneurysms
Capillary Degeneration
Endothelial To Pericyte Ratios
Mouse Eye
Retinal Blood Vessels
Retinal Blood Vessel Architecture
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