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一个孤立的半完整的上下游鼠标前庭上皮细胞电生理和高分辨率的双光子显微镜
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Neuroscience
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JoVE Journal Neuroscience
An Isolated Semi-intact Preparation of the Mouse Vestibular Sensory Epithelium for Electrophysiology and High-resolution Two-photon Microscopy
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一个孤立的半完整的上下游鼠标前庭上皮细胞电生理和高分辨率的双光子显微镜

DOI:
10.3791/50471-v

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11:52 min

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June 13, 2013

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, , , ,
1Discipline of Biomedical Science, School of Medical Sciences, Sydney Medical School,University of Sydney, 2School of Biomedical Sciences and Pharmacy,University of Newcastle

Chapters

  • 00:05Title
  • 02:23Preparation of Mouse Vestibular Sensory Epithelium
  • 06:11Electrophysiology
  • 07:44Two-photon Microscopy
  • 09:09Results: Electrophysiological Recordings and Two-photon Calcium Imaging of the Semi-intact Mouse Vestibular Sensory Epithelium
  • 11:21Conclusion

Summary

Automatic Translation

Automatic Translation

前庭毛细胞功能分析是复杂的，最难的部分颅骨，颞骨岩部内位置较深。功能最全的毛细胞的研究已经用毛细胞急性分离。在这里，我们描述了一个半完整的小鼠前庭上皮细胞的电生理和双光子显微镜研究编制。

Tags

VestibularHair CellsSensory EpitheliumElectrophysiologyTwo-photon MicroscopySemi-intact PreparationMouseCellular FunctionVestibular DysfunctionSynaptic ConnectionsSupporting Cells

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