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胚胎心室血管内皮细胞的分离
JoVE Journal
Biology
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JoVE Journal
Biology
Isolation of Embryonic Ventricular Endothelial Cells
Please note that all translations are automatically generated.
Click here for the English version.
胚胎心室血管内皮细胞的分离
DOI:
10.3791/50463-v
•
08:05 min
•
July 20, 2013
•
Laura A. Dyer
,
Cam Patterson
1
McAllister Heart Institute
,
University of North Carolina at Chapel Hill
Chapters
00:05
Title
01:01
Preparing Antibody-conjugated Beads and Collagen Plates
02:18
Excising and Digesting Heart
04:05
Isolating Endothelial Cells
06:21
Representative Endothelial Cell Isolation Results
07:36
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
原代细胞培养是一个非常有用的技术分析特定的细胞群体,在特定的发展阶段,特别是从转基因小鼠胚胎。这里,胚胎脑室解剖的和离解,抗体共轭的有孔玻璃珠的识别和分离出来的血管内皮细胞进行进一步的分析。
Tags
Embryonic Ventricular Endothelial Cells
Cell Culture
Primary Cells
Transgenic Mouse
Magnetic Beads
Cell Isolation
Trypsin
Developing Coronary Vasculature
Embryonic Stages
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