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胚胎心室血管内皮细胞的分离
JoVE Journal
Biology
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JoVE Journal Biology
Isolation of Embryonic Ventricular Endothelial Cells
DOI:

08:05 min

July 20, 2013

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Chapters

  • 00:05Title
  • 01:01Preparing Antibody-conjugated Beads and Collagen Plates
  • 02:18Excising and Digesting Heart
  • 04:05Isolating Endothelial Cells
  • 06:21Representative Endothelial Cell Isolation Results
  • 07:36Conclusion

Summary

Automatic Translation

原代细胞培养是一个非常有用的技术分析特定的细胞群体,在特定的发展阶段,特别是从转基因小鼠胚胎。这里,胚胎脑室解剖的和离解,抗体共轭的有孔玻璃珠的识别和分离出来的血管内皮细胞进行进一步的分析。

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