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Immunology and Infection
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一种简单有效的方法,通过流式细胞仪检测核转录因子激活人体中性粒细胞
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Immunology and Infection
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JoVE Journal
Immunology and Infection
A Simple and Efficient Method to Detect Nuclear Factor Activation in Human Neutrophils by Flow Cytometry
Please note that all translations are automatically generated.
Click here for the English version.
一种简单有效的方法,通过流式细胞仪检测核转录因子激活人体中性粒细胞
DOI:
10.3791/50410-v
•
08:26 min
•
April 09, 2013
•
Erick García-García
,
Eileen Uribe-Querol
,
Carlos Rosales
1
Department of Biological Sciences
,
University of Alberta
,
2
División de Estudios de Posgrado e Investigación, Facultad de Odontología
,
Universidad Nacional Autónoma de México
,
3
Department of Immunology, Instituto de Investigaciones Biomédicas
,
Universidad Nacional Autónoma de México
Chapters
00:05
Title
01:25
Isolation of Neutrophils (PMN) from Human Blood
03:02
Activating PMN
04:03
Isolation and Fixation of Nuclei
04:51
Nuclei Immunolabeling for Flow Cytometry Analysis
06:15
Flow Cytometry Analysis
06:49
Results: Monitoring NF-κB Dynamics
07:54
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
中性粒细胞是白细胞在血液中含量最丰富的。中性粒细胞具有转录调控功能,如生产的炎性细胞因子和抑制细胞凋亡。这些函数可以用这里介绍的方法,它允许核因素,通过流式细胞术检测和定量在孤立的核研究
Tags
Neutrophils
Leukocytes
Phagocytosis
Reactive Oxygen Species
Cytokines
Apoptosis
Transcription
Flow Cytometry
Nuclear Factors
NF-κB
Elk-1
Article
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