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Journal
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Immunology and Infection
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从脐带血分离的前体B细胞亚群
JoVE Journal
Immunology and Infection
This content is Free Access.
JoVE Journal
Immunology and Infection
Isolation of Precursor B-cell Subsets from Umbilical Cord Blood
Please note that all translations are automatically generated.
Click here for the English version.
从脐带血分离的前体B细胞亚群
DOI:
10.3791/50402-v
•
14:06 min
•
April 16, 2013
•
Md Almamun
,
Jennifer L. Schnabel
,
Susan T. Gater
,
Jie Ning
,
Kristen H. Taylor
1
Department of Pathology and Anatomical Sciences
,
University of Missouri-Columbia
,
2
Laboratory for Infectious Disease Research
,
University of Missouri-Columbia
Chapters
00:05
Title
02:12
Isolation of Mononuclear Cells from Umbilical Cord Blood
04:19
Modified B-cell Isolation from Mononuclear Cells Using MACS Separation
06:32
Antibody Labeling and Preparation for Cell Sorting
08:32
Cell Sorting Using the MoFlo XDP Flow Cytometer
11:07
Results: Isolation of Cord Blood Pre-cursor B-cell Subsets
13:13
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
在这里,我们描述的协议子集的前体B细胞从脐带血中隔离开来。阿足够的数量和质量,可从细胞中提取核酸,并且用于在随后的实验,利用DNA或RNA。
Tags
Umbilical Cord Blood
Hematopoietic Progenitor Cells
Precursor B-cells
B-cell Differentiation
Cell Isolation
Cell Sorting
Cell Surface Markers
Mononuclear Cells
Biotin
Fluorescent Labeling
Downstream Nucleic Acid Assays
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