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Uitvoering Dynamic Klem met Synaptic en kunstmatige geleidingen Mouse in retinale ganglioncellen
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Implementing Dynamic Clamp with Synaptic and Artificial Conductances in Mouse Retinal Ganglion Cells
DOI:

11:46 min

May 16, 2013

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Chapters

  • 00:05Title
  • 02:05General Set Up and Tissue Preparation
  • 05:32Patching Cell Bodies of Retinal Ganglion Cells
  • 07:20Recordings of Ganglion Cells Using Dynamic Clamp
  • 09:12Antibody Staining Against Lucifer Yellow Filled Retinal Ganglion Cells
  • 10:14Results: Implementing Dynamic Clamp with Synaptic and Artificial Conductances in Mouse Retinal Ganglion Cells
  • 11:27Conclusion

Summary

Automatic Translation

Deze video artikel illustreert de set-up, de procedures te cellichamen patchen en hoe u dynamische clamp opnames implementeren van ganglioncellen in zijn geheel-mount muis retinae. Deze techniek maakt het onderzoek de exacte rol prikkelende en remmende synaptische inputs, en hun relatieve omvang en timing neuronale spiking.

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