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制备胰腺腺泡细胞钙成像的目的，细胞损伤的测量，以及腺病毒感染
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JoVE Journal Biology
Preparation of Pancreatic Acinar Cells for the Purpose of Calcium Imaging, Cell Injury Measurements, and Adenoviral Infection
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制备胰腺腺泡细胞钙成像的目的，细胞损伤的测量，以及腺病毒感染

DOI:
10.3791/50391-v

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10:39 min

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July 05, 2013

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, , , , ,
1Rangos Research Center, Pediatric Gastroenterology, Hepatology, and Nutrition,Children’s Hospital of Pittsburgh of UPMC, 2Department of Surgery,Tufts University Medical Center

Chapters

  • 00:05Title
  • 01:53Preparing Pancreatic Acinar Cells for Calcium Imaging
  • 04:09Measuring Calcium in Pancreatic Acinar Cells
  • 05:08Preparation of Pancreatic Acinar Cells for Cell Injury Assays
  • 06:30Measuring Lactate Dehydrogenase Leakage
  • 07:33Infecting Pancreatic Acinar Cells with Adenovirus
  • 08:01Results
  • 09:52Conclusion

Summary

Automatic Translation

Automatic Translation

我们描述一个可重复的方法准备从小鼠小鼠胰腺腺泡细胞研究生理和病理相关刺激腺泡细胞钙信号和细胞损伤的目的。这些细胞中腺病毒感染的方法也被提供。

Tags

Pancreatic Acinar CellsCalcium ImagingCell InjuryAdenoviral InfectionExocrine PancreasPancreatitisLactate DehydrogenaseCell IsolationCell TransfectionCell PhysiologyCell Pathology

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