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糖尿病CD4の養子移入+ T細胞によるマウスの加速された1型糖尿病の誘導
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Accelerated Type 1 Diabetes Induction in Mice by Adoptive Transfer of Diabetogenic CD4+ T Cells
DOI:

06:27 min

May 06, 2013

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Chapters

  • 00:05Title
  • 01:11Isolation of T Cells from Spleen and Lymph Nodes of BDC2.5 Mice
  • 02:52Fluorescence-activated Cell Sorting of Diabetogenic CD4+ T Cells from BDC2.5 Mice
  • 04:28Adoptive Transfer of Diabetogenic CD4+ T Cells from BDC2.5 Mice
  • 05:12Monitoring Recipient Mice for Hyperglycemia and T1D
  • 05:36Results: Adoptive T-cell Transfer
  • 06:10Conclusion

Summary

Automatic Translation

私たちは、膵島抗原特異、プライマリーCD4 + T細胞の養子移入によって2週間以内に、マウスにおける1型糖尿病(T1D)を誘導するための再現可能な方法を提供する。

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