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一个<em在体外</em>模型研究人类巨噬细胞分化和极化的异质性
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
An In vitro Model to Study Heterogeneity of Human Macrophage Differentiation and Polarization
DOI:

07:42 min

June 12, 2013

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Chapters

  • 00:05Title
  • 01:09PBMC Isolation (Histopaque) and Cell Counting
  • 02:55Negative Isolation of Monocytes
  • 05:09Results: Representative Primary Human Macrophages
  • 07:15Conclusion

Summary

Automatic Translation

单核细胞源性巨噬细胞是先天免疫系统的重要细胞。这里,我们描述一个易于使用的<em>在体外</em>模型来生成这些细胞。使用梯度离心法,负胎圈分离和特定的细胞培养条件,可以产生单核细胞源性巨噬细胞表型和功能的研究。

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