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鉴定转基因鱼的完整的大脑中的神经元的电活动记录
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Recording Electrical Activity from Identified Neurons in the Intact Brain of Transgenic Fish
DOI:

07:45 min

April 30, 2013

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Chapters

  • 00:05Title
  • 01:27Dissection of Brains from Adult Medaka
  • 02:56Mounting the Brain in a Recording Chamber
  • 03:37Electrophysiology: Loose Patch Recording of Action Potential Firing
  • 05:36Electrophysiology: Whole Cell Recording of Membrane Potential
  • 06:26GnRH3 Neuron Activity in Medaka and Zebrafish
  • 07:27Conclusion

Summary

Automatic Translation

在这段视频中,我们将演示如何记录保留复杂的神经回路在整个大脑的准备,从确定单个神经元的电活动。我们使用转基因鱼中,促性腺激素释放激素(GnRH)的神经元与用于识别完整的大脑制备一种荧光蛋白基因的标签。

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