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鉴定转基因鱼的完整的大脑中的神经元的电活动记录
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Recording Electrical Activity from Identified Neurons in the Intact Brain of Transgenic Fish
Please note that all translations are automatically generated.
Click here for the English version.
鉴定转基因鱼的完整的大脑中的神经元的电活动记录
DOI:
10.3791/50312-v
•
07:45 min
•
April 30, 2013
•
Yali Zhao
,
Nancy L. Wayne
1
Department of Physiology
,
University of California, Los Angeles
Chapters
00:05
Title
01:27
Dissection of Brains from Adult Medaka
02:56
Mounting the Brain in a Recording Chamber
03:37
Electrophysiology: Loose Patch Recording of Action Potential Firing
05:36
Electrophysiology: Whole Cell Recording of Membrane Potential
06:26
GnRH3 Neuron Activity in Medaka and Zebrafish
07:27
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
在这段视频中,我们将演示如何记录保留复杂的神经回路在整个大脑的准备,从确定单个神经元的电活动。我们使用转基因鱼中,促性腺激素释放激素(GnRH)的神经元与用于识别完整的大脑制备一种荧光蛋白基因的标签。
Tags
Transgenic Fish
GnRH Neurons
Medaka Fish
Terminal Nerve
Neuromodulatory
Spontaneous Action Potential Firing
Kisspeptin
Intact Brain
Neuron Identification
Electrophysiology
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