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的分散Presomitic中胚层细胞培养的斑马鱼分割时钟在单细胞成像代
JoVE Journal
Biology
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JoVE Journal
Biology
Generation of Dispersed Presomitic Mesoderm Cell Cultures for Imaging of the Zebrafish Segmentation Clock in Single Cells
Please note that all translations are automatically generated.
Click here for the English version.
的分散Presomitic中胚层细胞培养的斑马鱼分割时钟在单细胞成像代
DOI:
10.3791/50307-v
•
10:41 min
•
July 24, 2014
•
Alexis B. Webb
,
Daniele Soroldoni
,
Annelie Oswald
,
Johannes Schindelin
,
Andrew C. Oates
1
Max Planck Institute of Molecular Cell Biology and Genetics
Chapters
00:05
Title
01:39
Dissection Preparation
03:15
Presomitic Mesoderm Dissection and Dispersal
06:40
Imaging Dispersed Presomitic Mesodermal Cells
07:58
Image Processing of Acquired Time-lapse Movies
08:58
Representative Time-lapse Imaging Results
10:00
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
体节形成是有节奏的发育过程,从而在空间型态脊椎动物胚胎的体轴。以前,我们开发了使用荧光记者观察环状基因,推动这一进程的转基因斑马鱼线。在这里,我们培养从这些线和图像的振荡时间的推移
在体外
分散细胞。
Tags
Zebrafish
Segmentation
Presomitic Mesoderm
Somites
Segmentation Clock
Her1
Fluorescent Reporter
In Vitro Culture
Single-cell Imaging
Time-lapse Imaging
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