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Journal
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Immunology and Infection
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听话的哺乳动物细胞原生动物引发的细菌感染
JoVE Journal
Immunology and Infection
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JoVE Journal
Immunology and Infection
Tractable Mammalian Cell Infections with Protozoan-primed Bacteria
Please note that all translations are automatically generated.
Click here for the English version.
听话的哺乳动物细胞原生动物引发的细菌感染
DOI:
10.3791/50300-v
•
13:54 min
•
April 02, 2013
•
Samuel L. Drennan
,
Amrita Lama
,
Ben Doron
,
Eric D. Cambronne
1
Department of Molecular Microbiology & Immunology
,
Oregon Health & Science University
Chapters
00:05
Title
02:13
Preparation of Legionella pneumophila Cultures for Priming Stage Infections
04:14
Priming Stage Infection Using Acanthamoeba castellanii
07:13
Processing of Bacteria for Target Cell Stage Infection after the Priming Stage Infection
09:46
Experimental Analysis
11:14
CFU Plating
11:51
Representative Infection Results
13:23
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
这种技术提供了一种方法来收集,标准化和量化,自然原虫的宿主细胞培养的哺乳动物细胞感染前细胞内生长的细菌病原体。此方法可以被修改,以适应各种各样的起动阶段,以及靶细胞类型的宿主细胞。
Tags
Tractable
Mammalian Cell Infections
Protozoan-primed Bacteria
Intracellular Bacterial Pathogens
Legionella Pneumophila
Legionnaires’ Pneumonia
Acanthamoeba Castellanii
Virulence Factors
GFP-expressing Strains
Fluorescence Microscopy
Flow Cytometry
Colony Plating
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