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分离的小鼠胚胎面外胚层和间充质

JoVE Journal
Biology

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JoVE Journal Biology

Separation of Mouse Embryonic Facial Ectoderm and Mesenchyme

Please note that all translations are automatically generated. Click here for the English version.

分离的小鼠胚胎面外胚层和间充质

DOI:

10.3791/50248-v

•

08:36 min

•

April 12, 2013

•

Hong Li, Trevor Williams²

¹Department of Craniofacial Biology,University of Colorado Denver Anschutz Medical Campus, ²Department of Cell and Developmental Biology,University of Colorado Denver Anschutz Medical Campus

Chapters

00:05Title
01:34Dissecting CD-1 E10.5 Embryos
03:18Dispase II Treatment
04:13Separating the Facial Ectoderm and Mesenchyme
06:37Tissue Purity after Separation
07:26Conclusion

Summary

Automatic Translation

外胚层和间充质细胞的胚胎面部分离的协议。我们使用的是中性蛋白酶II对待整个胚胎首先，剖析整个面部突起，然后分离的面部外胚层和间充质细胞。

Tags

Facial Ectoderm Facial Mesenchyme Orofacial Clefts Facial Prominences Embryonic Development Mouse Model Dispase II Gene Expression Transcription Factors Chromatin Marks

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