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使用高通量形状的RNA二级结构预测
JoVE Journal
Biology
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JoVE Journal
Biology
RNA Secondary Structure Prediction Using High-throughput SHAPE
Please note that all translations are automatically generated.
Click here for the English version.
使用高通量形状的RNA二级结构预测
DOI:
10.3791/50243-v
•
13:42 min
•
May 31, 2013
•
Sabrina Lusvarghi*
1
,
Joanna Sztuba-Solinska*
1
,
Katarzyna J. Purzycka
,
Jason W. Rausch
,
Stuart F.J. Le Grice
1
RT Biochemistry Section, HIV Drug Resistance Program
,
Frederick National Laboratory for Cancer Research
Chapters
00:05
Title
01:40
RNA Folding
03:09
Chemical Modification of the RNA
04:33
Reverse Transcription
07:08
Preparation of Sequencing Ladder and Capillary Electrophoresis
09:55
ShapeFinder Software
11:15
Data Normalization
11:51
Results: RNA Structure Determination using RNA Structure Software
12:53
Conclusion
Summary
Automatic Translation
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Automatic Translation
的高通量选择性2'羟基的酰化反应分析的引物延伸(SHAPE)利用了一种新的化学探测技术,反转录,毛细管电泳确定结构的RNA二级结构预测软件从几百到几千个核苷酸,单核苷酸分辨率。
Tags
RNA Secondary Structure
High-throughput SHAPE
Chemical Probing
Reverse Transcription
Capillary Electrophoresis
ShapeFinder
RNAStructure
In Silico Prediction
Single Nucleotide Resolution
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