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Medicine
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使用反时相蛋白的阵列(RPPA)探索在个人的肾细胞癌蛋白表达的变化
JoVE Journal
Medicine
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JoVE Journal
Medicine
The Use of Reverse Phase Protein Arrays (RPPA) to Explore Protein Expression Variation within Individual Renal Cell Cancers
Please note that all translations are automatically generated.
Click here for the English version.
使用反时相蛋白的阵列(RPPA)探索在个人的肾细胞癌蛋白表达的变化
DOI:
10.3791/50221-v
•
12:22 min
•
January 22, 2013
•
Fiach C. O’Mahony
,
Jyoti Nanda
,
Alexander Laird
,
Peter Mullen
,
Helen Caldwell
,
Ian M. Overton
,
Lel Eory
,
Marie O’Donnell
5
,
Dana Faratian
,
Thomas Powles
,
David J. Harrison
2
,
Grant D. Stewart
1
Edinburgh Urological Cancer Group
,
University of Edinburgh
,
2
School of Medicine
,
University of St Andrews
,
3
Division of Pathology
,
University of Edinburgh
,
4
MRC Human Genetics Unit, MRC IGMM
,
University of Edinburgh
,
5
Department of Pathology
,
Western General Hospital
,
6
Breakthrough Breast Cancer Research Unit
,
University of Edinburgh
,
7
St Bartholomew’s Cancer Institute, Experimental Cancer Medicine Centre
,
Queen Mary University of London
Chapters
00:05
Title
01:39
Identification of Morphological and Molecular Tumor Heterogeneity
02:58
Protein Extraction from Tumor Samples
04:24
RPPA Printing
06:00
RPPA Protein Detection
09:27
Results: Pre- and Post-TKI-treated Renal Cell Cancer Show Differential Protein Expression
12:06
Conclusion
Summary
Automatic Translation
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Automatic Translation
RPPA支持数百个样品,印在硝酸纤维素幻灯片同时进行审讯,用荧光标记的抗体蛋白的表达。这项技术已被应用在肾透明细胞癌的药物治疗的非均质性研究的效果。
Tags
Reverse Phase Protein Arrays (RPPA)
Renal Cell Cancer
Protein Expression
Tumor Heterogeneity
Targeted Therapy
Tyrosine Kinase Inhibitor
Personalized Treatment
Antibody Validation
Proteomic Analysis
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