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体外红血细胞溶血含量的生物大分子药物的细胞内交货的评价的pH响应Endosomolytic代理
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Ex Vivo Red Blood Cell Hemolysis Assay for the Evaluation of pH-responsive Endosomolytic Agents for Cytosolic Delivery of Biomacromolecular Drugs
DOI:

11:12 min

March 09, 2013

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Chapters

  • 00:01Title
  • 00:26Introduction fo Endosomolysis and Hemolysis
  • 02:16Preparation of Buffers and Test Reagents
  • 03:14Blood Sample Preparation
  • 06:05Assay Setup and Quantification
  • 08:39Data Analysis
  • 09:00Representative Results
  • 09:25Discussion

Summary

Automatic Translation

溶血试验可作为一种快速,高通量筛选,药物传递系统的细胞相容性和内货物配送endosomolytic活动的。该试验测量作为环境pH值的函数的红细胞膜的破坏。

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