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结合免疫荧光和三维保存的间期核荧光原位杂交DNA研究在3D核组织的变化
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Combined Immunofluorescence and DNA FISH on 3D-preserved Interphase Nuclei to Study Changes in 3D Nuclear Organization
Please note that all translations are automatically generated.
Click here for the English version.
结合免疫荧光和三维保存的间期核荧光原位杂交DNA研究在3D核组织的变化
DOI:
10.3791/50087-v
•
13:55 min
•
February 03, 2013
•
Julie Chaumeil
,
Mariann Micsinai
2,3,4
,
Jane A. Skok
1
Department of Pathology
,
New York University School of Medicine
,
2
New York University Center for Health Informatics and Bioinformatics
,
3
NYU Cancer Institute
,
4
Department of Pathology and Yale Cancer Center
,
Yale University School of Medicine
Chapters
00:05
Title
01:27
DNA Probe Labeling with Fluorophores: Nick Translation
03:37
Probe Precipitation/Denaturation/Pre-annealing
05:13
3-dimensional DNA FISH
09:25
3-dimensional DNA FISH / Immuno-FISH
10:40
Combined Immunofluorescence and DNA FISH: Representative Results
12:56
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
在这里,我们描述了一个协议,用于同时检测组蛋白修饰免疫荧光法和DNA序列的DNA荧光原位杂交,然后通过的3D显微镜和分析(3D的免疫-DNA FISH)。
Tags
3D DNA FISH
3D Nuclear Organization
3D Confocal Microscopy
Immunofluorescence
Chromatin Modifications
Gene Loci
Chromosome Territories
Nuclear Architecture
Nuclear Proteins
Histone Variants
Transcription Machinery
Nuclear Sub-compartments
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