JoVE
JoVE
Faculty Resource Center
Research
Behavior
Biochemistry
Biology
Bioengineering
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
JoVE Journal
JoVE Encyclopedia of Experiments
JoVE Chrome Extension
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to your Course
Authors
Librarians
High Schools
About
Sign-In
Sign In
Contact Us
Research
JoVE Journal
JoVE Encyclopedia of Experiments
Education
JoVE Core
JoVE Science Education
JoVE Lab Manual
High Schools
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Close
Research
Behavior
Biochemistry
Bioengineering
Biology
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
Products
JoVE Journal
JoVE Encyclopedia of Experiments
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
Products
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to Your Course
Teacher Resources
Get in Touch
Instant Trial
Log In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Journal
/
Immunology and Infection
/
制备肿瘤抗原负载成熟树突状细胞免疫治疗
JoVE Journal
Immunology and Infection
A subscription to JoVE is required to view this content.
Sign in or start your free trial.
JoVE Journal
Immunology and Infection
Preparation of Tumor Antigen-loaded Mature Dendritic Cells for Immunotherapy
Please note that all translations are automatically generated.
Click here for the English version.
制备肿瘤抗原负载成熟树突状细胞免疫治疗
DOI:
10.3791/50085-v
•
08:40 min
•
August 01, 2013
•
Rachel Lubong Sabado
,
Elizabeth Miller
,
Meredith Spadaccia
,
Isabelita Vengco
,
Farah Hasan
,
Nina Bhardwaj
1
Cancer Institute
,
NYU Langone Medical Center
,
2
Infectious Diseases
,
NYU Langone Medical Center
Chapters
00:05
Title
01:09
Isolation and Cryopreservation of PBMCs
03:09
Day 0: Differentiation of Dendritic Cells from Monocytes
04:33
Day 5: Harvesting of Immature Dendritic Cells
05:21
Day 6: Maturation and Antigen Loading of Dendritic Cells
05:58
Day 7: Cryopreservation of Dendritic Cells
07:13
Results: Poly-ICLC Treatment Induces PBMC Differentiation into Functionally Mature DCs
08:14
Conclusion
Summary
Automatic Translation
English (Original)
العربية (Arabic)
中文 (Chinese)
Nederlands (Dutch)
français (French)
Deutsch (German)
עברית (Hebrew)
italiano (Italian)
日本語 (Japanese)
한국어 (Korean)
português (Portuguese)
русский (Russian)
español (Spanish)
Türkçe (Turkish)
Automatic Translation
用于产生在肿瘤免疫治疗中使用的大量的自体树突状细胞(DC)的最常用的方法进行说明。该方法使用的IL-4和GM-CSF分化的单核细胞的树突状。未成熟DC刺激才成熟,然后装入抗原注射到病人。
Tags
Tumor Antigen
Dendritic Cells
Immunotherapy
Poly-ICLC
TLR3 Agonist
Ex Vivo Preparation
GMP Guidelines
Peripheral Blood Mononuclear Cells
Monocytes
IL-4
GM-CSF
Keyhole Limpet Hemocyanin
Peptide Antigens
Controlled-rate Freezer
Article
Embed
ADD TO PLAYLIST
Usage Statistics
Related Videos
激活的B细胞CD40的小鼠模型
高效转染树突状细胞的无细胞的成熟优化的协议
小鼠肺树突状细胞的分离
从人类胸腺髓样树突状细胞和上皮细胞的分离
CAR性T细胞过继治疗的护理标准胶质母细胞瘤的上下文生成
性T细胞Transinfection从树突状细胞捕捉细菌
不成熟,成熟和耐受性树突状细胞的生成与代谢不同表型
基于CFSE-试验鼠皮肤树突状细胞的迁移习进引流淋巴结在与感染<em>牛分枝杆菌</em>卡介苗
人干扰素α超灵敏单分子阵列数字酶联免疫吸附试验的研制与验证
一种新的生理免疫原树突状细胞生成方法
Read Article