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Peptide: MHC Tetramero basata Arricchimento di epitopi specifici per cellule T
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Chapters
- 00:05Title
- 01:50Cell Isolation from Lymphoid Tissue
- 03:35Tetramer Staining
- 04:18Magnetic Enrichment
- 06:39Flow Cytometry
- 09:07Results: Calculation of MHC Tetramer-based Enrichment of Epitope-specific T Cells
- 13:15Conclusion
Questo protocollo descrive l'uso del peptide: MHC tetrameri e microsfere magnetiche per isolare popolazioni bassa frequenza di epitopo-specifiche cellule T e analizzarli mediante citometria a flusso. Questo metodo consente lo studio diretto delle popolazioni di cellule T endogene di interesse da<em> In vivo</em> Sistemi sperimentali.
Tags
Peptide:MHC Tetramer-based EnrichmentEpitope-specific T CellsT Cell Antigen Receptor SpecificityFunctional ResponseProliferationCytokine ProductionActivation MarkersTCR Transgenic Adoptive Transfer ModelMonoclonal T CellsHistocompatible HostsCongenic Marker AntibodyExperimental ArtifactsFunctional HeterogeneityPolyclonal Population
