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在毛发再生的上皮 - 间充质信号的快速遗传分析
JoVE Journal
Biology
This content is Free Access.
JoVE Journal
Biology
Rapid Genetic Analysis of Epithelial-Mesenchymal Signaling During Hair Regeneration
Please note that all translations are automatically generated.
Click here for the English version.
在毛发再生的上皮 - 间充质信号的快速遗传分析
DOI:
10.3791/4344-v
•
10:09 min
•
February 28, 2013
•
Wei-Meng Woo*
1
,
Scott X. Atwood*
1
,
Hanson H. Zhen
,
Anthony E. Oro
1
Program in Epithelial Biology
,
Stanford University School of Medicine
Chapters
00:05
Title
01:31
Isolating and Preparing Mouse Skins
03:17
Isolating Primary Dermal or Epidermal Cells from Skins
05:07
Grafting Primary Dermal and Epidermal Cells
07:57
Histological Analysis of Hair Follicle Growth
09:32
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
组织特异性分析的毛囊再生模型,利用慢病毒介导增益或损失的功能。
Tags
Genetic Analysis
Epithelial-mesenchymal Signaling
Hair Regeneration
Hair Follicle Morphogenesis
Organ Development
Lentivirus
Overexpression
ShRNA-mediated Knockdown
Gain-of-function Analysis
Loss-of-function Analysis
Primary Mouse Keratinocytes
Dermal Cells
Dermal Papilla Cells
Hair Follicles
Nude Mice
Genetic Models
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