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代人类诱导多能干细胞从外周血中使用STEMCCA慢病毒载体
JoVE Journal
Biology
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JoVE Journal
Biology
Generation of Human Induced Pluripotent Stem Cells from Peripheral Blood Using the STEMCCA Lentiviral Vector
Please note that all translations are automatically generated.
Click here for the English version.
代人类诱导多能干细胞从外周血中使用STEMCCA慢病毒载体
DOI:
10.3791/4327-v
•
12:03 min
•
October 31, 2012
•
Andreia Gianotti Sommer
,
Sarah S. Rozelle
,
Spencer Sullivan
,
Jason A. Mills
,
Seon-Mi Park
,
Brenden W. Smith
,
Amulya M. Iyer
,
Deborah L. French
,
Darrell N. Kotton
,
Paul Gadue
,
George J. Murphy
,
Gustavo Mostoslavsky
1
Center for Regenerative Medicine (CReM)
,
Boston University School of Medicine
,
2
Department of Hematology
,
Children’s Hospital of Philadelphia
,
3
Center for Cellular and Molecular Therapeutics
,
Children’s Hospital of Philadelphia
Chapters
00:05
Title
01:50
Isolation and Expansion of PBMCs
04:34
Transduction of PBMCs with STEMCCA Lentivirus
06:11
Plating Transduced Cells onto MEFs
08:41
Picking and Expansion of iPSC Clones
09:26
Results: Human Induced Pluripotent Stem Cells
11:26
Conclusion
Summary
Automatic Translation
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Automatic Translation
这里,我们表明一个简单而有效的协议,用于从外周血3-4毫升,使用一个单一的慢病毒重新编程矢量代人类iPSCs。现成的血细胞重新编程的承诺,加快利用IPSC的技术,使其接触到更广泛的研究团体。
Tags
Human Induced Pluripotent Stem Cells
Peripheral Blood
STEMCCA Lentiviral Vector
Transcription Factors
Oct4
Klf4
Sox2
CMyc
Pluripotent State
Embryonic Stem Cells
Immune Rejection
Disease Modeling Studies
Pharmacological Compounds Screening
Regenerative Therapies
Transgene-free IPSCs
Lung Diseases
Skin Fibroblasts
Reprogramming
Peripheral Blood Cells
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