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在Live哺乳动物细胞中质粒复制多代荧光显微镜监测
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Biology
Monitoring Plasmid Replication in Live Mammalian Cells over Multiple Generations by Fluorescence Microscopy
Please note that all translations are automatically generated.
Click here for the English version.
在Live哺乳动物细胞中质粒复制多代荧光显微镜监测
DOI:
10.3791/4305-v
•
12:34 min
•
December 13, 2012
•
Kathryn Norby
,
Ya-Fang Chiu
,
Bill Sugden
1
Department of Oncology
,
University of Wisconsin – Madison
Chapters
00:05
Title
01:34
Engineering of Cells with Visible Plasmids
05:08
Visualization of Labeled DNA
09:33
Post-acquisition Processing of Images
10:33
Results: Visualization of Plasmids in Live Mammalian Cells
12:16
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
观察单个活细胞中的DNA分子的方法,进行说明。该技术是基于对一个荧光标记的lac阻遏物蛋白质设计到感兴趣的DNA结合位点的结合。此方法可适于后续许多随着时间的推移在活细胞中的重组DNA。
Tags
Plasmid Replication
Live Mammalian Cells
Fluorescence Microscopy
Gamma-herpesviruses
Epstein-Barr Virus
Kaposi’s Sarcoma-associated Herpesvirus
Licensed Replication
Viral Protein
Cellular Replication Machinery
Centromeres
Southern Blotting
Fluorescence In Situ Hybridization (FISH)
Quantitative PCR
Visualizing Plasmids
Lactose Repressor Protein
LacO Sequence
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