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在Live哺乳动物细胞中质粒复制多代荧光显微镜监测
JoVE Journal
Biology
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JoVE Journal Biology
Monitoring Plasmid Replication in Live Mammalian Cells over Multiple Generations by Fluorescence Microscopy
DOI:

12:34 min

December 13, 2012

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Chapters

  • 00:05Title
  • 01:34Engineering of Cells with Visible Plasmids
  • 05:08Visualization of Labeled DNA
  • 09:33Post-acquisition Processing of Images
  • 10:33Results: Visualization of Plasmids in Live Mammalian Cells
  • 12:16Conclusion

Summary

Automatic Translation

观察单个活细胞中的DNA分子的方法,进行说明。该技术是基于对一个荧光标记的lac阻遏物蛋白质设计到感兴趣的DNA结合位点的结合。此方法可适于后续许多随着时间的推移在活细胞中的重组DNA。

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