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Journal
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Immunology and Infection
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枚举主要外周血白细胞人口多中心临床试验,使用全血表型分析
JoVE Journal
Immunology and Infection
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JoVE Journal
Immunology and Infection
Enumeration of Major Peripheral Blood Leukocyte Populations for Multicenter Clinical Trials Using a Whole Blood Phenotyping Assay
Please note that all translations are automatically generated.
Click here for the English version.
枚举主要外周血白细胞人口多中心临床试验,使用全血表型分析
DOI:
10.3791/4302-v
•
14:45 min
•
September 16, 2012
•
Tiffany R. Hensley
,
Austin B. Easter
,
Sarah E. Gerdts
,
Stephen C. De Rosa
,
Antje Heit
,
M. Juliana McElrath
,
Erica Andersen-Nissen
1
Vaccine and Infectious Disease Division
,
Fred Hutchinson Cancer Research Center
Chapters
00:00
Title
00:10
Background and Significance
02:08
Reagents
03:37
Preparation
05:04
Staining
07:54
Shipping
09:46
Analysis
10:51
Representative Data
13:42
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
在这份报告中,我们展示了染色和分析步骤,新鲜全血进行列举主要的先天免疫和适应性白细胞种群的表型分析。执行这些程序在多中心临床试验的背景下,我们强调的注意事项。
Tags
Peripheral Blood Leukocyte Populations
Multicenter Clinical Trials
Whole Blood Phenotyping Assay
Cryopreservation
Immune Response Evaluations
Granulocytes
B Cells
Dendritic Cells
Fresh Leukocytes Assay
Volunteer Commitments
Timeframes
Working Hours
Laboratory Personnel
Multiple Centers
11-color Antibody Staining Panel
Trucount Tubes
Phenotype Enumeration
Article
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