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膜片钳记录在内耳毛细胞的分离斑马鱼
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Patch Clamp Recordings in Inner Ear Hair Cells Isolated from Zebrafish
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膜片钳记录在内耳毛细胞的分离斑马鱼

DOI:
10.3791/4281-v

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12:43 min

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October 17, 2012

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, , , , , , ,
1Natural Science Division,Pepperdine University

Chapters

  • 00:05Title
  • 01:52Pre-experimental Preparations
  • 02:47Isolation of Auditory and Vestibular Labyrinth
  • 05:28Hair Cell Isolation
  • 07:18Patch Clamping Zebrafish Hair Cells
  • 10:12Results: Isolation of and Electrophysiological Recordings from Zebrafish Hair Cells
  • 12:04Conclusion

Summary

Automatic Translation

Automatic Translation

这个视频的目的是为了证明程序获得的成年斑马鱼的内耳器官的健康，完整的毛细胞，然后利用他们为特征的生物物理特性的电压门控离子通道的膜片钳研究的目的。

Tags

Patch Clamp RecordingsInner Ear Hair CellsZebrafishVoltage-gated ChannelsSensory Hair CellsIsolation MethodHair Cell SizeHair Cell MorphologyDiversityPatch Clamp RecordingsZebrafish Model SystemMutantsHearing And BalanceTransgenic LinesGenetic AnalysisElectrophysiological MethodsSensory Modalities

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